LONDON, UK (Radio 2) - The band's 1975 hit heads a list compiled to mark the 50th anniversary of the UK charts, to be broadcast by BBC
Radio 2 on Saturday night.
The operatic rock song, which regularly makes it to the top of listeners' charts, came in ahead of two other usual suspects - John Lennon's Imagine and Hey Jude by The Beatles - placed second and third.
Fellow former Beatle George Harrison's My Sweet Lord was placed at number five.
The Beatles as a group claim 14 of the top 100, way ahead of any other act. Only three of their number ones failed to make it into the list.
Contemporary stars barely get a look in, with only four tracks from the past decade making it into the entire top 100.
Only Kylie Minogue's Can't Get You Out Of My Head, Elvis's revamped A Little Less Conversation, Oasis's Don't Look Back In Anger and U2's Beautiful Day represent the past 10 years.
The most contemporary track in the first 25 is Band Aid's Do They Know It's Christmas, dating to 1984.
Bohemian Rhapsody last topped a major national poll in May based on more than 30,000 entries compiled by the Guinness World Records British Hit Singles book.
The track topped the charts twice, despite record label bosses at EMI initially being reluctant even to issue the single.
There have been 941 number one songs since the national UK pop charts began 50 years ago. The Official UK Charts Company, which compiles the weekly listing, compiled nearly 190,000 votes to draw up its definitive list.
The 50 highest were counted down in a Radio 2 show, The Greatest Number Ones Of All Time, by DJ Richard Allinson on Saturday 9 November.
The top 100 number one UK singles:
1. Queen - Bohemian Rhapsody
2. John Lennon - Imagine
3. Beatles - Hey Jude
4. Simon & Garfunkel - Bridge Over Troubled Water
5. George Harrison - My Sweet Lord
6. Procol Harum - A Whiter Shade Of Pale
7. Animals - The House Of The Rising Sun
8. Abba - Dancing Queen
9. Beach Boys - Good Vibrations
10. Queen & David Bowie - Under Pressure
11. Kate Bush - Wuthering Heights
12. Pink Floyd - Another Brick In The Wall
13. Police - Every Breath You Take
14. Righteous Brothers - You've Lost That Loving Feeling
15. Band Aid - Do They Know It's Christmas
16. Rolling Stones - (I Can't Get No) Satisfaction
17. Beatles - She Loves You
18. Soft Cell - Tainted Love
19. Beatles - All You Need Is Love
20. Elvis Presley - Jailhouse Rock
21. 10 CC - I'm Not In Love
22. Steve Harley & Cockney Rebel - Make Me Smile (Come Up And See Me)
23. Rod Stewart - Maggie May
24. Roy Orbison - Oh Pretty Woman
25. David Bowie - Space Oddity
26. Sinead O'Connor - Nothing Compares 2 U
27. Beatles - I Want To Hold Your Hand
28. Dexy's Midnight Runners - Come On Eileen
29. Marvin Gaye - I Heard It Through The Grapevine
30. Monkees - I'm A Believer
31. Boomtown Rats - I Don't Like Mondays
32. Beatles - A Hard Day's Night
33. Beatles - Help!
34. Frankie Goes To Hollywood - Relax
35. Kylie Minogue - Can't Get You Out Of My Head
36. Queen - Innuendo
37. Byrds - Mr Tambourine Man
38. Abba - Waterloo
39. Elvis vs JXL - A Little Less Conversation
40. Kinks - You Really Got Me
41. Oasis - Don't Look Back In Anger
42. Elvis Presley - All Shook Up
43. Fleetwood Mac - Albatross
44. Elvis Presley - Are You Lonesome Tonight
45. Bryan Adams - (Everything I Do) I Do It For You
46. Rolling Stones - Paint It Black
47. Nilsson - Without You
48. Elvis Presley - The Wonder Of You
49. Louis Armstrong - What A Wonderful World/Cabaret
50. Ian Dury & The Blockheads - Hit Me With Your Rhythm Stick
51. Abba - The Winner Takes It All
52. Blondie - Heart Of Glass
53. Beatles - Day Tripper/We Can Work It Out
54. Elvis Presley - Can't Help Falling In Love/Rock-A-Hula Baby
55. George Michael - Careless Whisper
56 Beatles - Ticket To Ride
56 John Lennon - Woman
58. Beatles - Can't Buy Me Love
59. Elvis Presley - It's Now Or Never
60. Jimi Hendrix - Voodoo Chile
61. Dusty Springfield - You Don't Have To Say You Love Me
62. Beatles - Yellow Submarine/Eleanor Rigby
63. Human League - Don't You Want Me
64. Roy Orbison - Only The Lonely
65. Bill Haley & His Comets - Rock Around The Clock
66. Beatles - Paperback Writer
67. Jam - Going Underground
68. T Rex - Get It On
69. Slade - Merry Xmas Everybody
70. Sonny & Cher - I Got You Babe
71. Kinks - Sunny Afternoon
72. Bonnie Tyler - Total Eclipse Of The Heart
73 Joe Cocker - With A Little Help From My Friends
73 Abba - Mamma Mia
75 Gerry & The Pacemakers - You'll Never Walk Alone
75 David Bowie - Ashes To Ashes
75 Righteous Brothers - Unchained Melody
78. Beatles - Get Back
79. Abba - Knowing Me Knowing You
80. Madonna - Like A Prayer
81. Elvis Presley - Return To Sender
82. Bangles - Eternal Flame
83 Freddie Mercury - Living On My Own
83 U2 - Beautiful Day
85. Buggles - Video Killed The Radio Star
86. Rolling Stones - Jumping Jack Flash
87. Meat Loaf - I'd Do Anything For Love (But I Won't Do That)
88. Michael Jackson - Billie Jean
89. Scott McKenzie - San Francisco (Be Sure To Wear Some Flowers In Your Hair)
90. Beatles - From Me To You
91. Alice Cooper - School's Out
92. A-ha - The Sun Always Shines On TV
93. Bee Gees - Night Fever
94. Gloria Gaynor - I Will Survive
95 Rolling Stones - Honky Tonk Women
95 Hollies - He Ain't Heavy He's My Brother
97. Beatles - Hello Goodbye
98. Smokey Robinson & The Miracles - Tears Of A Clown
99 Police - Message In A Bottle
99 Billy Joel - Uptown Girl